流式细胞术资源

1.Cytek极光5激光
  1. 20mw, 355 nm (UV) - 16通道检测器，UV1 - UV16, 365-829 nm
  2. 100mw, 405nm(紫色)- 16通道检测器，V1 - V16, 420-829 nm
  3. 50mw, 488 nm(蓝色)- 14通道探测器，B1 - B14, 498-829 nm
  4. 50mw, 561 nm(黄/绿)- 10通道检测器，UG1 - UG10, 567-829 nm
  5. 80mw, 640 nm(红色)-8通道检测器，R1 - R8, 653-829 nm
2.ZE5电池分析仪，5个激光器:

1.相干OBIS BSO 355nm 50mW激光器:

A. 690dlp, 700lp (buv737)

B. 560DLP, 670/30BP (BUV661, Hoechst 33342-Red, PI)

C. 493dlp, 525/50bp (buv496, buv 563)

D. 405DLP, 447/60BP (Alexa350, DAPI, Hoechst 33342-Blue, Indo-1 low， -Ca++）

E.无，387/11BP (BUV395, Indo-1 high， + ca++)

2.相干OBIS BSO 405nm 100mW激光器:

A. 752dlp, 750lp (bv786)

B. 690dlp, 720/60bp (bv711)

C. 640dlp, 670/30bp (bv650)

D. 581dlp, 615/24bp (bv605)

E. 493DLP, 525/50BP (AmCyan, BV510，级联黄，太平洋橙)

F. 439DLP, 460/22BP (Pacific Blue, Horizon BV450, DAPI)

G.无，420/10BP (Cascade Blue, BV421, Alexa405)

3.相干OBIS BSO, 488nm 100mW激光器，具有:

A. 752DLP, 750LP (PE-Cy7, PE-Alexa750)

B. 629DLP, 692/80BP (PE-Cy5, PerCP- cy5.5, PerCP, PE-Cy5.5, PE-Alexa647)

C. 558DSP, 593/52BP (PE, PE- texas Red, PI)

D. 493DLP, 525/35BP (eGFP, FITC, Alexa488)

E.无，488/10BP (SSC)

4.相干OBIS BSO 561nm 50mW激光器

A. 750DSP, 750DLP (PE-Cy7, PE-Alexa750)

B. 690DLP, 720/60BP (PE-Cy-5.5)

C. 652DLP, 670/30BP (PE-Alexa647, PE-Cy5)

D. 629DLP, 640/20BP (mPlum)

E. 600DLP, 615/24BP (mCherry, PE-Texas Red, PI, Texas Red)

F. 581dlp, 589/15bp ()

G.无，577/15BP (dTomato, dsRed, PE)

5.相干OBIS BSO 640nm 100mW激光器:

A. 800dlp, 800lp ()

B. 752DLP, 775/50BP (APC-Cy7, APC-H7, APC-Alexa750)

C. 690DLP, 720/60BP (Cy5.5, Alexa680, Alexa700)

D.无，670/30BP (APC, Cy5, Alexa647)

3.lsrii新型四激光细胞分析仪:

1.相干蓝宝石固态488nm, 20mW激光器:

A. 685LP, 710/50BP (PerCP-Cy5.5)

B. 505LP, 525/50BP (Alexa488, FITC, GFP, CFSE, YFP)

C.无，488/10BP (SSC)

2.相干COMPass黄绿561nm, 25mW激光器:

A. 740LP, 780/60BP (PE-Cy7, PE-Alexa750)

B. 685LP, 710/50BP (PE-Alexa680, PE-Cy5.5)

C. 635LP, 660/20BP (PE-Cy5, PE-Alexa647, 7AAD)

D. 600LP, 610/20BP (PI, Alexa594, HCred, mCherry, mRFP1, PE-Texas Red, Alexa568, PE-CF594)

E. 570LP, 582/15BP (DsRed, PE, DsRed2, dTomato,Cy3)

3.JOS单相1344p HeNe红633nm, 40mW激光器:

A. 740LP, 780/60BP (APC-Cy7, APC-Alexa750, APC-H7)

B. 685LP, 710/50BP (Alexa680, APC-Cy5.5, Alexa700)

C.无，670/30BP (APC, Alexa647, Cy5)

4.相干Vioflame固态紫罗兰407nm, 50mW激光器，具有:

A. 740LP, 780/60BP (Qdot800)

B. 685LP, 705/70BP (Qdot705)

C. 565LP, 610/20BP (Qdot605, BV605)

D. 505LP, 525/50BP (Alexa430, CFP, Qdot565,AmCyan, HorizenV500)

E.无，450/50BP (Alexa405, Cascade-Blue, PacificBlue, eFluor450, HorizenV450, BV421, CFP)

4.LSRII细胞分析仪与三个激光:

1.相干蓝宝石固态488nm, 20mW激光器:

A. 755LP, 780/60BP (PE-Cy7)

B. 685LP, 695/40BP (PerCP- cy5.5, PerCP)

C. 655LP, 670/14BP (PE-Cy5，尼罗红，7AAD)

D. 600LP, 610/20BP (PE-德州红，碘化丙啶，二氢乙二，PE- CF594)

E. 550LP, 575/26BP (PE, PI, DsRed, Dil)

F. 505LP, 530/30BP (FITC, Alexa488, GFP, SYBR绿色，YFP, CFSE)

G.无。488/10BP (SSC)

2.JDS单相HeNe 633nm, 17mW激光器

A. 755LP, 780/60BP (APC-Cy7, APC-H7, Alexa750)

B. 710LP, 730/45BP (Alexa700)

C.无，660/20BP (APC, Cy5, DiD)

3.点源紫固态407nm, 25mW激光器，具有:

A. 505LP, 525/50BP (Alexa430, AmCyan, horizon v500, CFP)

B.无，450/50BP(太平洋蓝，Alexa405，海洋蓝，CFP, coumarine Hcc2, eFluor450, BV421)

5.FACSAriaIII细胞分选与五个激光

1.UV Genesis 355nm 50mW激光器:

A. 690lp, 740/35bp (buv737)

B. 410LP, 450/50BP (Indo-1 Violet, DAPI)

C.无，379/28BP (BUV395)

2.紫罗兰立方体405nm 100mW与:

A. 750LP, 780/60BP (BV786, Qdot800)

B. 690LP, 710/50BP (BV711, Qdot700)

C. 655LP, 670/30BP (Qdot605, Brilliant Violet (BV650))

D. 595LP, 610/20BP (BV605, Qdot605)

E. 505LP, 525/50 (AmCyan, V500, BV510)

F.无、450/40 (BV421、太平洋蓝、V450)

3.蓝色蓝宝石488nm 100mW与:

A. 655LP, 695/40BP (7aad, PerCp- cy5.5, PerCp)

B. 502LP, 530/30BP (Alexa488, FITC, GFP, BB515, CFSC, YFP)

C.无，488/10BP (SSC)

4.黄绿色罗盘561nm 110mW与:

A. 735P, 780/60BP (Pe-Cy7)

B. 685LP, 710/50BP (PE-Cy5.5)

C. 600LP, 610/20BP (PE-CF594, PE-TEXAS RED, PI, Alexa 594, mCherry)

D.无，582/15BP (dsRed, PE, PE- cy5, Alexa 568。dTomatto)

5.Red CUBE 633nm 40mW带有:

A. 755LP, 780/60BP (APC-Cy7, APC-H7)

B. 690LP, 730/45BP (APC - Alexa700, APC- r700)

C.无，660/20BP (APC, Alexa647)

6.FACSAria-FUSION四激光器。

蓝色、黄绿色和红色既可以与紫色搭配，也可以与nUV搭配

1.488nm -蓝- 50mW(相干蓝宝石):

A. 655LP, 695/40BP (695/40-B, PerCP -cy5.5,PerCP)

B. 502LP, 530/30BP (530/30-B, FITC, Alexa 488, Brilliant Blue515, GFP, CFSE, YFP, SYBR Green)

2.561nm -黄-绿- 50 mW(相干Sappier):

A. 735LP, 780/60BP (780/60-YG, PE-Cy7, PE-Alexa750)

B. 685LP, 710/50BP (710/50-YG, PE-Cy5.5, PE-Alexa680)

C. 630LP, 670/14BP (670/14-YG, PE-Cy5, 7AAD, PE-Alexa647)

D. 600LP, 610/20BP (610/20-YG, PI, PE-CF594, PE-TxRed, Alexa594, mCherry, mRFP1,Alexa568)

E.无，582/15BP (582/15-YG, PE, DsRed, dTomato, Cy3)

3.640 nm -红- 100mW(相干Obis):

A. 755LP, 780/60BP (780/60-R, APC-Cy7, APC-H7, APC-Alexa750)

B. 690LP, 730/45BP (730/45-R, Alexa700)

C.无，670/30BP (670/30-R, APC, Alexa647, Cy5)

4.405nm - violet - 85mW (Point Source Iflex 2000):

A. 750LP, 780/60BP (780/60-V, BV786, Qdot800)

B. 690LP, 710/50BP (710/50-V, BV711, Qdot705)

C. 630lp, 660/20bp (660/20 v, bv660)

D. 595LP, 610/20BP (610/20-V, BV605, Qdot605)

E. 505LP, 525/50BP (525/50-V, AmCyan, Horizon V500, BV510, Alexa430)

F.无，450/50BP (450/50-V，太平洋蓝，BV421, Alexa405, Cascade-Blue, eFluor450, CFP, DAPI-Violet)

7.FACSAriaIII单元分选机，四束激光:

1.相干蓝光488nm 20mW激光器:

A. 655LP, 695/40BP (PerCP - cy5.5, PerCP)

B. 502LP, 530/30BP (FITC, CFSE, GFP, YFP, Alexa488)

C.无，488/10BP (SSC)

2.相干黄绿色561nm 40mW激光器

A. 735LP, 780/60BP (PE-Cy7, PE-Alexa750)

B. 685LP, 710/50BP (PE-Cy5.5, PE-Alexa680)

C. 630LP, 670/14BP (PE-Cy5, PE-Alexa647, 7AAD)

D. 600LP, 610/20BP (PI, PE-TxRed, Alexa594, mCherry, HCred, mRFP1, PE-CF594)

E.无，582/15BP (PE, DsRed, Alexa568, dTomato, DsRed2)

3.JDSU HeNeRed 633 nm 20mW激光器

A. 755LP, 780/60BP (APC-Cy7, APC-Alexa750, APC-H7)

B. 690LP, 730/45BP (APC-Cy5.5, Alexa700)

C.无，660/20BP (APC, Cy5, Alexa647)

4.相干紫罗兰407nm 50mW与:

A. 735lp, 780/60bp (qdot800)

B. 690LP, 710/50BP (BV711, Qdot705, eFluor 700NC)

C. 630LP, 660/20BP (BV650, Qdot655, eFluor 650NC, eVolve650)

D. 595LP, 610/20BP (BV605, Qdot605, eFluor 605NC, eVolve605)

E. 502LP, 510/50BP (Alexa430, CFP, qd525, AmCyan, horizon V500, CFP)

F.无，450/ 40bp (Alexa405，太平洋蓝，Cascade蓝，eFluor 450, horizon V450, BV421, CFP)

8.SORP FACSAriaII细胞分选机，5个激光:

1.UV创世纪355nm 65mW激光器:

A. 505LP, 530/30BP (Hoechst红，Indo-1蓝)

B.无，450/50BP (Alexa350, Hoechst, Indo-1 Violet, DAPI-UV)

2.紫罗兰立方体405nm 100mW与:

A. 750LP, 780/60BP (Qdot800)

B. 670LP, 705/70BP (Qdot705)

C. 595LP, 605/40BP (Qdot605, Brilliant Violet605)

D. 505LP, 525/50BP (Alexa430, AmCyan, CFP, Qdot525, horizon V500, CFP)

E.无，450/50 (Alexa405，级联蓝，太平洋蓝，DAPI-紫罗兰，CFP, horizon V450, BV421)

3.蓝色蓝宝石488nm 100mW与:

A. 685LP, 710/50BP (PerCp-Cy5.5)

B. 505LP, 525/50BP (Alexa488, FITC, GFP, CFSE, YFP)

C.无，488/10BP (SSC)

4.黄绿罗盘561nm 110mW与:

A. 750LP, 780/60BP (Pe-Cy7, PE-Alexa750)

B. 685LP, 710/50BP (PE-Alexa680, PE-Cy5.5)

C. 635LP, 660/20BP (PE-Cy5, PE-Alexa647)

D. 600LP, 610/20BP (PE- texas Red, PI, Alex 594, HCred,mCherry, mRFP1, PE- CF594)

E.无，582/15BP (DsRed, PE, DsRed2-Alexa568, dTomato)

5.Red CUBE 640nm 40mW带有:

A. 750LP, 780/60BP (APC-Cy7, APC-Alexa750, APC-H7)

B. 685LP, 710/50BP (Alexa680, Alexa700, APC-Cy5.5)

C.无，670/14BP (APC, Alexa647, Cy5)

9.带有三个激光的FACSMelody细胞分选机:

1.紫罗兰405nm二极管激光器，40mW与:

A. 755LP，无(BV786)

B. 500LP, 528/45BP (V500, BV510, Pacific Orange, AmCyan)

C.无，448/45BP (V450, Pacific Blue, DAPI, BV421)

2.蓝色488nm二极管激光器，20mW带:

A. 752LP, 783/56BP (PE-Cy7, PE-Alexa750)

B. 665LP, 700/54BP (PE-Cy5.5, PerCP, PerCP- cy5.5, 7-AAD, BB700)

C. 560lp, 586/42bp (pe, pi)

D. 507LP, 527/32BP (FITC, GFP, BB515, Alexa488, CFSE)

E.无，488/15BP (SSC)

3.红色640nm二极管激光器，40mW带:

A. 752LP, 783/56BP (APC-Cy7, APC-Alexa750, APC-H7)

B.无，660/10BP (APC, Alexa647, Cy5)

10.FACSymphony S6细胞分选5激光
  1. 紫外355nm, 100mW激光，具有:

    A. 770 lp, 810/40 bp (buv805)

    B. 690 lp, 735/30 bp (buv737)

    C. 630 lp, 670/20 bp (buv661)

    D. 600 lp, 610/20 bp (buv615)

    E. 550 lp, 580/20 bp (buv563)

    F. 490 lp, 515/30 bp (buv496)

    G. 410 LP, 450/50 BP (DAPI, UV440, Hoechst, AlexaFluor350, Indo-Violet)

    H. 370 lp, 379/28 bp (buv395)

  2. 紫罗兰405nm, 200mW激光器，具有:

    A. 770 lp, 810/40 bp (bv786)

    B. 735 lp, 750/30 bp (bv750)

    C. 685 lp, 710/50 bp (bv711)

    D. 635 lp, 677/20 bp (bv650)

    E. 595 lp, 605/40 bp (bv605)

    F. 550 lp, 585/15 bp (bv570)

    G. 505 LP, 525/50 BP (BV480, BV510, V500, AlexaFluor430, CFP, AmCyan)

    H. 410 LP, 431/28 BP (BV421, V450，太平洋蓝，AF405)

  3. 蓝色488nm, 200Mw激光器，具有:

    A. 770 lp, 810/40 bp (bb790)

    B. 735 lp, 750/30 bp (bb755)

    C. 685 LP, 710/50 BP (BB700, PerCP, PerCP- cy5.5)

    D. 635 lp, 670/30 bp (bb660)

    E. 600 lp, 610/20 bp (bb630)

    F. 505 lp, 530/30 bp (bb515, fitc, gfp, yfp, af488, cfsc)

    G.无，488/10 BP (SSC)

  4. 黄绿561nm, 200mW激光带:

    A. 750 LP, 780/60 BP (PE-Cy7, PE-Alexa750)

    B. 690 LP, 710/50 BP (PE-Cy5, 7AAD)

    C. 595 LP, 610/20 BP (PE-CF594, PE-Texas Red, mCherry, PI, AF594)

    D. 570 LP, 586/15 BP (PE, DsRed, tdTomato)

  5. 红色637nm, 140mW激光器，具有:

    A. 750 LP, 780/60 BP (APC-H7, APC-Cy7, APC-Alexa750)

    B. 690 LP, 730/45 BP (APC-R700, AF700, Red718)

    C. 655 LP, 670/30 BP (APC, AF647,Cy5)

11.ImageStreamX流量成像仪系统，5个激光器:

1.激发激光器:

A.紫外光405nm, 120mW

B.蓝色488 nm 100 mW

C.黄绿色561 nm, 200 mW

D.红色642 nm 150 mW

E.暗场激光785 nm (SSC)。

2.发射通道(nm):

A. 430-480: Brightfield

B. 480-560: fitc, gfp, af488, yfp

C. 560-595: PE, Cy3, AF546, AF555

D. 595-660: RFP, PI, mCherry, AF568

E. 660-745:百分比，PE-Cy5, PE-AF647

F. 745-800: PE-Cy7, PE-AF750, SSC

G. 430-505: DAPI, Hoechst，太平洋蓝，CFP, A405

H. 505-570: PacOrange, AF430, QD525

I. 570-595: Brightfield

J. 595-640: QD625, eFluor625

K. 640-745: APC, Cy5, AF647, Draq5, PerCP

L. 745-800: QD800, APC-Cy7,APC -AF750,Cy7, AF750

3.ImageSreamX包含:

A.两个摄像头，12个成像通道

B.放大20倍、40倍和60倍

C.扩展景深光学

D.单色亮场

E. 96孔板自动采样器

F.使用IDEAS软件的数据分析工作站。

12.ImageStream-NEW流量成像仪系统与5个激光

1.激发激光器:

A.紫外光405 nm 120mW

B.蓝色488nm 400 mW

C.黄绿色561nm 200 mW

D.红色642 nm 150 mW

E. 785 nm暗场激光(SSC)

2.发射通道(nm):

A. 430-480: Brightfield

B. 480-560: FITC, GFPm AF488, YFP

C. 595-660: RFP, PI, mCherry, AF568

D. 660-745:百分比，PE-Cy5, PE-AF647

E. 745-800: PE-Cy7, PE-AF750, SSC

F. 430-505: DAPI, Hoechst，太平洋蓝，CFP, A405

G. 505-570: PacOrange, AF430, QD525

H. 570-595: Brightfield

I. 595-640: QD625, eFluor625

J. 640-745: APC, Cy5, AF647, Draq5, PerCP

K. 745-800: QD800, APC-Cy7, APC-AF750, Cy7,AF750

3.ImageStreamX包含:

A.两个摄像头，12个成像通道

B.放大20倍、40倍和60倍

C.单色亮场

D.使用IDEAS软件的数据分析工作站

13.Helios细胞计数仪
14.MIBI质量成像
15.EVOS FL自动2成像系统

A.全自动倒立多通道荧光透射光成像系统。

B.配有恒温CO2恒温培养箱。

C.可选镜头:4X、20X。

D.可用通道-亮场和绿色(GFP, FITC, AF488)

额外的设备
  • 一台带有FlowJo, FCS-express和Modfit的PC机，用于离线分析。
  • An_Heraeus_Labofuge 400离心机。
  • heraeus_孵化器HERA细胞。
  • NUAIR生物安全柜。
  • 地板接地，避免静电。
  • 正压单台空调。温度维持在22-24摄氏度之间。
  • 中央空气压力出口允许高达80 PSI。
  • 真空泵。
  • 荧光室照明和额外的变阻器控制。
  • 无反射表面的墙壁和水槽。

安全:
实验室入口处的危险标志。
当激光对准时打开的红灯。
位于操作员附近的激光主断开。
电锁房间用磁卡进入